CAT#:
11-735
Zymo Research
ZymoScript One-Step RT-qPCR Kit
Single Tube cDNA Synthesis and qPCR, 101 Reactions/Unit
Product Overview
- Flexible RT-qPCR kit compatible with both, SYBR Green and TaqMan based assays
- Efficient cDNA synthesis and robust amplification in as little as 1.5 hours
- Superior performance
- Contains blue tracking dye to help reaction set-up
Technical Specifications
| Sample Source | RNA |
| Storage Condition | -80C |
Detailed Description
ZymoScript One-Step RT-qPCR Kit is an optimized RT-qPCR kit that contains all the necessary components for fast reverse transcription and robust qPCR amplification in a single tube. The One-Step RT-qPCR kit is compatible with both SYBR Green and TaqMan probe-based assays for ultimate flexibility. The visual tracking dye included in the master mix allows accurate reaction set-up.
Citations
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Frequently Asked Questions
What is the minimum/maximum amount of RNA template I can use?
The amount of total RNA required may vary depending on the expression level of the target transcripts. In general, we recommend using 0.1 pg - 5 µg of input RNA.
Will the blue tracking dye interfere with qPCR fluorophore channels and affect the signal of probe/fluorescent dyes?
The dye used in ZymoScript RT PreMix has been thoroughly tested in downstream analyses. At the provided concentration, it will not affect fluorescent signal readings of any qPCR fluorescent channel.
How is the performance of ZymoScript RT with low-quality RNA samples?
Lower cDNA yields can be expected with degraded and/or impure RNA samples. We recommend using Quick-RNA or Direct-zol Kits (see Related Products) for high quality RNA extraction.
Can I use oligo dT or random hexamer primers?
No, only use gene specific primers when using ZymoScript One-Step RT-qPCR Kit.
Can I assemble the RT reaction at room temperature?
Yes, the reaction can be assembled at room temperature. The enzymes are only activated at higher temperatures, ensuring maximum specificity.
Can I store the ZymoScript One-Step RT-qPCR kit at Room Temperature or 4°C?
We do not recommend storing the kit or kit components at room temperature for prolonged periods of time. The kit can be stored at 4°C for up to 1 week.
How can I ensure that there is no genomic DNA contamination in my RT reaction?
Samples can be treated with DNase I to eliminate DNA contamination. DNase I is included in the Quick-RNA or Direct-zol Kits recommended for RNA extraction. Alternatively, the DNase I set (see Related Products) can be purchased separately.
Do I need to add RNase inhibitors?
No, RNase inhibitors are already included in the enzyme mix to prevent unwanted RNA degradation during reverse transcription.