(11-728) Mix & Go! XJb Autolysis Compet Zymo Research 10 x 100µl/Unit
(11-728) Mix & Go! XJb Autolysis Compet Zymo Research 10 x 100µl/Unit
CAT#: 11-728 Zymo Research

Mix & Go! XJb Autolysis Competent Cells

1ml 500x L-Arabinose, 10 x 100µl/Unit

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Product Overview
  • Fast: 80 - 90% of E. coli are lysed in only 10 minutes after harvesting.
  • High Transformation Efficiencies: Achieve 108 - 109 transformants per µg of plasmid DNA.
  • Versatile: Fully compatible with a wide range of buffers for protein purification and other physical methods of lysis.
Technical Specifications
BrandZymo Research Corporation
AutolysisXJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the lysis buffer. However, even very low concentrations of detergent may improve lysis significantly.
Cell GrowthA very robust strain, reaching higher OD’s than E. coli K-strains.
DNA ExtractionXJb is not optimal for DNA extraction.
DNA StabilityThis strain is RecA positive.
Transformation Efficiency108 - 109 transformants per µg of plasmid DNA
Processing Time10 minutes
Product Storage-70°C to -80°C
Protein ExpressionXJb is ideal for recombinant protein expression. It lacks Lon and OmpT proteases, leading to higher protein yields.
Detailed Description


While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage lambda R gene, encoding the lambda lysozyme, also known as lambda endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.
Citations

Frequently Asked Questions

Are the Mix & Go! strains dam+ and dcm+?
Most cloning strains will be dam+/dcm+ unless specifically noted in the genotype.
Can glucose be added to the growth media?
When glucose is added to the growth media, it inhibits the induction of the autolysis genes when it is present in the media. As the cells grow, they consume the glucose as a carbon source. Once the glucose has been consumed autolysis begins.
Can glycerol be present during the freeze-thaw cycle?
Do not perform the freeze and thaw cycle in a buffer containing glycerol. Glycerol protects the E.coli from forming ice crystals which are essential to the lysis of the cells.
How do you improve lysis efficiency?
If the results obtained are not satisfactory, lysis can be significantly improved by incubating the cells at higher temperatures (25 - 37°C) or for longer time (10 or 20 minutes) after thawing (step 5).
Do heat shock and outgrowth steps have to be performed when transforming XJb Autolysis E. coli?
It is necessary for high transformation efficiencies. However, if your experiment does not require very high transformation efficiency (e.g. when using plasmid stock to transform E. coli), incubate the DNA and cells on ice for 1-5 minutes and spread directly onto prewarmed plates
Are competent cells GMOs?
All our competent cells are classified into Biosafety level 1 and are not genetic modified organisms. Only when transformed with a plasmid they become GMOs.

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