Cat #: 11-708
Zymo Research E2018-50 dsDNA Shearase Plus, Zymo Research, 50U/Unit
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Cat #: 11-708
Zymo Research E2018-50 dsDNA Shearase Plus, Zymo Research, 50U/Unit
Zymo Research
50U/Unit
Brand: Zymo Research- The simplest method for generating random-ended dsDNA fragments
- Fragment size can be controlled by adjusting the enzyme concentration
- Fragments generated using dsDNA Shearase Plus are ideal for library construction, NGS, methylated DNA immunopreciptation (MeDIP, MeDIP-Seq), etc
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$155.50
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Zymo Research
50U/Unit
Brand: Zymo Research- The simplest method for generating random-ended dsDNA fragments
- Fragment size can be controlled by adjusting the enzyme concentration
- Fragments generated using dsDNA Shearase Plus are ideal for library construction, NGS, methylated DNA immunopreciptation (MeDIP, MeDIP-Seq), etc
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3'-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
Brand | Zymo Research Corporation |
---|---|
Concentration | 1U/µl |
Enzyme Inactivation | Heat inactivate enzyme at 65°C for 5 min. |
Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at </= -70°C. |
Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250ng human DNA into DNA fragments in the range of 100-500bp in 20 minutes at 42°C in total reaction volume in 10µl. |
Yes, we observe that there is more T in the second base. Only the first and second base are a little imbalanced, but the nucleotide distribution is balanced from the 3rd base onward.
Here are some possible reasons: The dsDNA Shearase Plus is specific for dsDNA and does not work efficiently for ssDNA. The performance of the enzyme is significantly affected by RNA contamination present in the samples. Check the quality of your DNA sample as DNA/RNA hybridization might occur during the 42°C incubation. Make sure that enzyme is scaled up or down properly in the reaction.
The enzyme can be inactivated by incubation at 65°C for 5 minutes. The DNA Clean & Concentrator-5 (D4003) can be used to clean up the reaction.
Yes, please follow the manufacturers protocol. You can also use the Select-a-Size DNA Clean & Concentrator MagBead Kit.
dsDNA Shearase Plus is dsDNA specific, thus, RNA will remain intact if there is RNA contamination in the samples. Hybridization between DNA and RNA might occur in RNA contaminated samples, reducing the performance and efficiency of the fragmentation. We recommend using RNA-free DNA as a substrate.
Enzymatic activity is not affected by methylation. Enzymes have been tested with human, mouse, and plant DNA and the performance was the same.
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