Brand | Zymo Research Corporation |
Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
Elution Volume | >/= 37.5µl DNA Elution Buffer or DNase free water |
Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block |
Processing Volume | Fecal </=100 mg, Soil </= 200 mg, Cells </= 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
Sample Storage | Eluted DNA should be stored at </= -20°C. |
Size Range | Typically 15-20kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
Type | Total DNA |
Yield | Up to 10µg total DNA can be eluted into 50µl (37.5µl minimum) |