Cat #: 11-616C

Zymo Research D6430-PS3 Quick-16S Plus NGS Library Prep Kit (V4), w/ Primer Set 3, 96 Rxns/Unit

Zymo Research D6430-PS3 Quick-16S Plus NGS Library Prep Kit (V4), w/ Primer Set 3, 96 Rxns/Unit Primary Image
Zymo Research D6430-PS3 Quick-16S Plus NGS Library Prep Kit (V4), w/ Primer Set 3, 96 Rxns/Unit Primary Image
Zymo Research D6430-PS3 Quick-16S Plus NGS Library Prep Kit (V4), w/ Primer Set 3, 96 Rxns/Unit Primary Image

Cat #: 11-616C

Zymo Research D6430-PS3 Quick-16S Plus NGS Library Prep Kit (V4), w/ Primer Set 3, 96 Rxns/Unit


w/ Primer Set 3

96 Rxns/Unit

Brand: Zymo Research

  • 8x less hands-on time than conventional 16S library prep protocols
  • Premixed plate minimizes hands-on time and handling errors
  • 100% automation ready with only a single PCR step and no need for normalization
  • Only 30 minutes of hands-on time for 96 samples

Guest Price:
$1,164.00
List Price: $1,200.00
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w/ Primer Set 3

96 Rxns/Unit

Brand: Zymo Research

  • 8x less hands-on time than conventional 16S library prep protocols
  • Premixed plate minimizes hands-on time and handling errors
  • 100% automation ready with only a single PCR step and no need for normalization
  • Only 30 minutes of hands-on time for 96 samples

The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ?388 bp.
Equipment Needed (user provided) Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates.
Index Sequences 10 bp indexes are listed in the MiSeq Sample Sheet Template provided.
Sample Input Sequencing Platform Purified microbial DNA (?100 ng), free of PCR inhibitors. Compatible with all Illumina sequencing platforms using custom sequencing primers. We recommend the MiSeq Reagent Kit v2 (300-cycle).
V4 Primer Sequences (adapters not included) 515f (GTGYCAGCMGCCGCGGTAA) and 806r (GGACTACNVGGGTWTCTAAT).
V4 Premix Plate Each position of the premix plate contains Equalase qPCR Premix, water, and uniquely indexed (UDI) 515f and 806r V4 primers mixed at their proper ratios for a 10 µl final reaction volume.

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