CAT#:
11-600MT
Zymo Research
ZymoBIOMICS96 MagBead DNA Kit (Lysis Rack)
Lysis Rack (96-Well), 2 x 96 Preps/Unit
Product Overview
- High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluid
- Innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc
- The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps
- Elution Volume: = 37.5 µl DNA Elution Buffer or DNase free water
- Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield
- Sample Storage: Eluted DNA should be stored at = -20ºC.
- Yield: Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)
- Equipment: Centrifuge with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or robotic sample processor, 96 well plate heat block
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume | ≥ 37.5 μl DNA Elution Buffer or DNase free water |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block |
| Processing Volume | Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Supplemental Info | How to Use Microbial Community Standards Tips & Tricks to Reduce Bioburden Score More Spore DNA |
| Type | Total DNA |
| Yield | Up to 10 μg total DNA can be eluted into 50 μl (37.5 μl minimum) |
Detailed Description
The ZymoBIOMICS® DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS® lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™.This kit is equipped with our OneStep™ PCR Inhibitor removal technology, enabling PCR from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Sample Sources: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from mammalian feces, soil, fungal/bacterial cells, biofilms and water. Elution Volume: = 37.5 µl DNA Elution Buffer or DNase free water. Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield™. Equipment: Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block. Sample Storage: Eluted DNA should be stored at = -20ºC.. Purity: High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing. Yield: Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum). Processing Volume: Fecal =100 mg, Soil = 200 mg, Cells = 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells). Type: Total DNA. Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Sample Sources: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from mammalian feces, soil, fungal/bacterial cells, biofilms and water. Elution Volume: = 37.5 µl DNA Elution Buffer or DNase free water. Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield™. Equipment: Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block. Sample Storage: Eluted DNA should be stored at = -20ºC.. Purity: High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing. Yield: Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum). Processing Volume: Fecal =100 mg, Soil = 200 mg, Cells = 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells). Type: Total DNA. Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
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