The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 3.0 mg of high-quality plasmid DNA with less than 0,025 EU/ug of DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for multiple applications including transfection, CRISPR, gene modification, restriction endonuclease digestion, lentiviral vectors, synthetic biology, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.