Autolysis | XJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the lysis buffer. However, even very low concentrations of detergent may improve lysis significantly. |
Cell Growth | A very robust strain, reaching higher OD’s than E. coli K-strains. |
DNA Extraction | XJb is not optimal for DNA extraction. |
DNA Stability | This strain is RecA positive. |
Genotype | F- ompT hsdSB(rB - mB -) gal dcm ΔaraB::ΛR, cat (CmR) |
Processing Time | 10 minutes |
Product Storage | -70°C to -80°C |
Protein Expression | XJb is ideal for recombinant protein expression. It lacks Lon and OmpT proteases, leading to higher protein yields. |