(11-475A) DNase I Set 250 U w/ Reaction Buffer 1 Set/Unit
(11-475A) DNase I Set 250 U w/ Reaction Buffer 1 Set/Unit
(11-475A) DNase I Set 250 U w/ Reaction Buffer 1 Set/Unit
(11-475A) DNase I Set 250 U w/ Reaction Buffer 1 Set/Unit
CAT#: 11-475A Zymo Research

DNase I Set 250 U

w/ Reaction Buffer, 1 Set/Unit

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Product Overview
  • Provided lyophilized (250 U) with DNA Digestion Buffer (4 ml)
  • Inactivation: 65°C for 10 min
Technical Specifications
1unit DefinitionOne Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
2storageAfter reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
3reconstitutionAdd DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
4inactivationHeat inactivate at 75 °C for 10 min with 5 mM EDTA. const tableHeaders = document.querySelectorAll('.spec-table th'); tableHeaders.forEach(header => { header.textContent = header.textContent.substring(1) })
Detailed Description
DNase I cuts both double-stranded and single-stranded DNA, producing 3'-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion.

Unit Definition: One unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.001 A260 units/min/ml of reaction mixture at 25°C (pH 5.0). (Kunitz, M. (1950) J Gen Physiol 33, 349 and 363)

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