Cat #: 11-411C
Zymo Research D4001T Zymoclean Gel DNA Recovery Kit (Uncapped), Zymo Research, 10 Preps/Unit
Cat #: 11-411C
Zymo Research D4001T Zymoclean Gel DNA Recovery Kit (Uncapped), Zymo Research, 10 Preps/Unit
Zymo Research
10 Preps/Unit
Brand: Zymo Research- Quick recovery of ultra-pure DNA from agarose gels
- Column design permits DNA elution at high concentrations into minimal volumes
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc
$35.25
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Zymo Research
10 Preps/Unit
Brand: Zymo Research- Quick recovery of ultra-pure DNA from agarose gels
- Column design permits DNA elution at high concentrations into minimal volumes
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc
The Zymoclean Gel DNA Recovery Kit provides rapid purification of high quality DNA from TAE/TBE-buffered agarose gels and features Zymo-Spin technology to yield high-quality, purified DNA in just minutes. Zymo-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Gel DNA Recovery kits is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
| Brand | Zymo Research Corporation |
|---|---|
| Applicable For | Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
| Elution Volume | >/= 6µl of DNA Elution Buffer |
| Equipment | Microcentrifuge |
| Purity | A260/A280 >1.8, A260/A230>1.8 |
| Sample Source | DNA excised from TAE/TBE-buffered agarose gels. |
| Size Range | 50bp to 23kb |
| Yield | Typically, up to 5µg total DNA per column can be eluted with >/=6µl water. For DNA 50bp to 10kb the recovery is 70-90%. For DNA 11kb to 23kb the recovery is 50-70% |
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Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Zymoclean has been validated for use with Zymo-Spin IIC and IIN columns. Other columns with higher binding capacity have not been validated by Zymo Research but have successfully been used by users.
Yes. Zymo-Spin I/IC Columns and DNA Wash Buffer are interchangeable.
Add 4 volumes of Agarose Dissolving Buffer for agarose gels >2% and ensure the gel slice has been fully dissolved.
No, high efficiency recovery is not guaranteed and the success of the purification can be variable.
If only isolating ssDNA, Zymo Research recommends the Zymoclean Gel RNA Recovery Kit. With some modification of the Zymoclean Gel DNA Recovery protocol, it is possible to recover ssDNA: After gel dissolving (step 3), add half a volume of isopropanol. Continue with the protocol.
Up to 400 mg of agarose gel.
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