Cat #: 11-397A
Zymo Research D4068T Quick-DNA Miniprep Plus Kit, Zymo Research, 10 Preps/Unit
Cat #: 11-397A
Zymo Research D4068T Quick-DNA Miniprep Plus Kit, Zymo Research, 10 Preps/Unit
Zymo Research
10 Preps/Unit
Brand: Zymo Research- Quick & Easy: Simple 20 minute procedure
- Highest Yield: Recover 3x more DNA
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc
$60.75
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Zymo Research
10 Preps/Unit
Brand: Zymo Research- Quick & Easy: Simple 20 minute procedure
- Highest Yield: Recover 3x more DNA
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc
For high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
| Brand | Zymo Research Corporation |
|---|---|
| Elution Volume | >/= 35µl |
| Equipment | Water bath or heat block (55°C), microcentrifuge, and vortex. |
| Purity | High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230>/= 2.0). |
| Size Range | Capable of recovering genomic and mitochondrial DNA sized fragments > 50kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| Workflow | Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA. |
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Q1: What is the difference between the digestion buffers? Can one buffer be used for all sample types?
The BioFluid & Cell Buffer (Red) is designed to allow for rapid Proteinase K digestion with easy-to-lyse samples (e.g. mammalian cells and biological fluids). The Solid Tissue buffer (Blue) can be used for any sample type and requires a longer PK digestion time compared to using the BioFluid & Cell Buffer (Red).
The Quick-DNA is optimized for cells, soft tissues, and homogenized/digested samples using a single lysis/binding buffer. The Quick-DNA Plus kits contain an optimized Proteinase K for processing a wider variety of sample inputs, such as cells, blood, tissues, etc. The upgraded Quick-DNA Plus recovers more DNA with higher purity compared to the Quick-DNA Kits.
Yes, samples can be digested overnight. Make sure to follow the appendix (page 8 appendix B) for processing liquids samples in DNA/RNA Shield and incubate at room temperature.
White blood cells, which are the major source of genomic DNA in blood, easily and quickly settles. Mix the blood sample well prior to aliquoting for purification.
E.coli cells are easy-to-lyse and can be processed directly with the Biological Fluids & Cells protocol. For an all-inclusive kit with any type of microbes (including tough-to-lyse), use any of Zymo Research’s Environmental Kits (e.g. Quick-DNA Fungal/Bacterial, Quick-DNA Fecal/Soil, ZymoBIOMICS DNA, etc.).
No, the kit is designed for direct use with biological samples. For clean-up of previously isolated DNA, please use the Genomic DNA Clean & Concentrator or the DNA Clean & Concentrator kits.
Beta-mercaptoethanol is a reducing agent that helps break down proteins and improves DNA recovery and purity. Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM) or omitted.
Yes, add 4 volumes of Genomic Lysis Buffer to 1 volume of crude lysate, homogenized, or digested sample (see Cell Suspensions and Proteinase K Digested Samples) and proceed with the remainder of the protocol.
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