(11-394B) Micrococcal Nuclease Zymo Research (10 U/100µl)/Unit
(11-394B) Micrococcal Nuclease Zymo Research (10 U/100µl)/Unit
(11-394B) Micrococcal Nuclease Zymo Research (10 U/100µl)/Unit
CAT#: 11-394B Zymo Research

Micrococcal Nuclease

Zymo Research, (10U/100µl)/Unit

List Price: $55.46
List Price: $55.46
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Technical Specifications
BrandZymo Research Corporation
Concentration0.1U/µl
Enzyme Commission Number(E.C. 3.1.31.1)
Enzyme InactivationEDTA or EGTA in molar excess of CaCl2.
StorageStore at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be </= -70 °C.
Unit DefinitionOne unit (U) will produce 1.0µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.
Detailed Description
Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3'-phosphates.

Frequently Asked Questions

Is there information on whether the exonuclease activity of the MNase digest dsDNA from 3' to 5'?
MNase is an endo-exonuclease that preferentially cleaves at AT or AU-rich regions, yielding mono-nucleotides and oligonucleotides with terminal 3'-phosphates.

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