Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).

Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.

No, tissues do not need to be homogenized.

Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.

Yes, sort directly into DNA/RNA Shield (e.g. Collect 100µl of FACS sorted cells into minimally 400µl of DNA/RNA Shield).

DNA & RNA (<-20°C): Indefinitely

RNA > 1 month DNA > 2 years