CAT#:
Zymo Research
EZ DNA Methylation Kits
MagPrep, 8 x 96 Rxns/Unit
MagPrep, 4 x 96 Rxns/Unit
Zymo Research Kit, 50 Rxns/Unit
Spin Column Format, 50 Preps/Unit
Shallow-Well, 2 x 96 Rxns/Unit
Zymo Research Kit, 2 x 96 Rxns/Unit
Zymo Research, 200 Rxns/Unit
7 product variations: View Options
Product Overview
- Proven procedure for complete bisulfite conversion of DNA.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Product Overview
- Proven procedure for complete bisulfite conversion of DNA.
- High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Product Overview
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Product Overview
- A complete system for DNA methylation detection!
- DNA bisulfite treatment, robust hot-start PCR, and a universally methylated human control DNA standard with primers
- Designed for the first time user requiring a consolidated product to perform DNA methylation analysis
- Conversion Efficiency: > 99.5%
- Processing Time: 4 hrs
- DNA Recovery: > 80%
- Spin column format
Product Overview
- Streamlined, proven procedure for bisulfite conversion of DNA
- Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc
- DNA Recovery > 80%
- Binding Capacity: 5 µg/well
- Processing Time: 12 - 16 hrs
- Conversion Efficiency: >99%
Product Overview
- Streamlined, proven procedure for bisulfite conversion of DNA
- Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc
- DNA Recovery > 80%
- Binding Capacity: 5 µg/well
- Processing Time: 12 - 16 hrs
- Conversion Efficiency: >99%
Product Overview
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Technical Specifications
| Conversion | > 99% |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | > 70% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | > 99% |
| Equipment | Thermocycler with heated lid, heating element for 96-well plate, magnetic stand. |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | > 70% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥10 μl |
| Equipment | Microcentrifuge and thermocycler with heated lid |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | >80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
Technical Specifications
| Applicable For | Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc. |
| Conversion | > 99.5% |
| Elution Volume | ≥ 10 μl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | Samples containing between 50 pg to 2 μg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells. |
| Processing Time | 4 hours |
| Recovery | > 80% |
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 15 μl for Deep-well ≥ 30 μl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | >80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥ 15 μl for Deep-well ≥ 30 μl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | >80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info | Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link |
Technical Specifications
| Conversion | >99% |
| Elution Volume | ≥10 μl |
| Equipment | Microcentrifuge and thermocycler with heated lid |
| Input | 500 pg - 2 μg of DNA |
| Processing Time | 12-16 hours |
| Recovery | >80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Technical Documents
Detailed Description
The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Citations
Powered by Bioz
7 Product Options
Catalog Number:
11-334MB
List Price:
$2569
Your Price:
$2202
Specifications:
| Quantity | 8 x 96 Reactions - MagPrep |
| Description | MagPrep |
| Qty/Unit | 8 x 96 Rxns/Unit |
Catalog Number:
11-334M
List Price:
$1608.06
Your Price:
$1378.34
Specifications:
| Quantity | 4 x 96 Reactions - MagPrep |
| Description | MagPrep |
| Qty/Unit | 4 x 96 Rxns/Unit |
Catalog Number:
11-334
List Price:
$375.66
Your Price:
$321.99
Specifications:
| Quantity | 50 Reactions |
| Description | Zymo Research Kit |
| Qty/Unit | 50 Rxns/Unit |
Catalog Number:
11-334SK
List Price:
$897.58
Your Price:
$769.36
Specifications:
| Quantity | 50 Preps |
| Description | Zymo Research |
| Qty/Unit | 50 Preps/Unit |
Catalog Number:
11-334S
List Price:
$1017.21
Your Price:
$871.9
Specifications:
| Quantity | 2 x 96 Reactions - Shallow Well |
| Description | Shallow-Well |
| Qty/Unit | 2 x 96 Rxns/Unit |
Catalog Number:
11-334HT
List Price:
$855.36
Your Price:
$733.17
Specifications:
| Quantity | 2 x 96 Reactions - Deep Well |
| Description | Deep Well Format |
| Qty/Unit | 2 x 96 Rxns/Unit |
Catalog Number:
11-334B
List Price:
$1077.18
Your Price:
$923.31
Specifications:
| Quantity | 200 Reactions |
| Description | Zymo Research |
| Qty/Unit | 200 Rxns/Unit |
| Catalog # | Description | Qty/Unit | Quantity | Price (USD) | Quantity | Add to Order | |
|---|---|---|---|---|---|---|---|
| 11-334 | Zymo Research Kit | 50 Rxns/Unit | 50 Reactions | ||||
| 11-334B | Zymo Research | 200 Rxns/Unit | 200 Reactions | ||||
| 11-334HT | Deep Well Format | 2 x 96 Rxns/Unit | 2 x 96 Reactions - Deep Well | ||||
| 11-334M | MagPrep | 4 x 96 Rxns/Unit | 4 x 96 Reactions - MagPrep | ||||
| 11-334MB | MagPrep | 8 x 96 Rxns/Unit | 8 x 96 Reactions - MagPrep | ||||
| 11-334S | Shallow-Well | 2 x 96 Rxns/Unit | 2 x 96 Reactions - Shallow Well | ||||
| 11-334SK | Zymo Research | 50 Preps/Unit | 50 Preps |
Need help?
Call our product expert?
+1.800.789.5550
or
Contact Us
7:00am - 5:00pm PST
Monday - Friday
!