Product Overview
- Recovery of purified RNA from a wide range of fungus species using Fast-Spin column technology
- Omits the use of glass beads and organic denaturants
- Eluted RNA is suitable for use in RT-PCR or other RNA-based procedures
- Sample sources: Fungi susceptible to yeast lytic enzyme lysis
- Processing Time: 30 min
Technical Specifications
| Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications |
| Elution Volume | ≥ 60 µl DNase/RNase-Free Water |
| Equipment | Microcentrifuge & Heat Block/Bath |
| Processing Time | 30 minutes |
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107 cells) |
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | ≥ 17 nt |
| Type | Total RNA |
| Yield | ≤ 25 μg of total RNA |
Technical Documents
Detailed Description
The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
Citations
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