Product Overview
- Purify high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, and solid tissues
- Zymo-Spin™ Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
- Sample sources: Up to 3 ml of biological fluids, 3 x 107 mammalian/cell cultures, or 125 mg of solid tissue.
- The DNA binding capacity of the column is 125 µg.
- DNA can be eluted into as little as 200 µl DNA Elution Buffer or water.
Technical Specifications
| Elution Volume | ≤ 200 μl |
| Equipment | Water bath or heat block (55°C), centrifuge or vacuum source and manifold, microcentrifuge, vortex, conical tubes (15 – 50 ml), and microcentrifuge tubes. |
| Purity | High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0). |
| Size Range | Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| Supplemental Info | Purpose of Genomic DNA Extraction & Tips For Success |
| Workflow | Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA. |
| Yield | The DNA binding capacity of the column is 125 μg. Typically, mammalian tissues yield: 1-3 μg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 μg DNA per mg liver and kidney. Human whole blood will yield 3-7 μg DNA per 100 μl blood sampled. |
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