Cat #: 11-302B
Zymo Research D4003T DNA Clean & Concentrator-5 (Uncapped), Zymo Research, 10 Preps/Unit
Cat #: 11-302B
Zymo Research D4003T DNA Clean & Concentrator-5 (Uncapped), Zymo Research, 10 Preps/Unit
Zymo Research
10 Preps/Unit
Brand: Zymo Research- Clean and concentrate up to 5µg DNA with </= 6µl elution in as little as 2 minutes with 0µl wash residue carryover
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc
$35.25
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Zymo Research
10 Preps/Unit
Brand: Zymo Research- Clean and concentrate up to 5µg DNA with </= 6µl elution in as little as 2 minutes with 0µl wash residue carryover
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc
The DNA Clean & Concentrator-5 provides purification of up to 5µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
| Brand | Zymo Research Corporation |
|---|---|
| Detergent Tolerance | </=5% Triton X-100, </=5% Tween-20, </=5% Sarkosyl, </=0.1% SDS |
| Elution Volume | >/= 6µl |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8 |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50bp to 23kb |
| Yield | </= 5µg total DNA can be recovered. For DNA 50bp to 10kb the recovery is 70-95%. For DNA 11kb to 23kb the recovery is 50-70%. |
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Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Working with volumes below 50µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100µl with water to ensure optimal binding conditions.
Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
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