The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.

Working with volumes below 50µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100µl with water to ensure optimal binding conditions.

Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.