(11-254) Alpha-Factor Mating Pheromone Zymo Research 240 µl/Unit
(11-254) Alpha-Factor Mating Pheromone Zymo Research 240 µl/Unit
CAT#: 11-254 Zymo Research

Alpha-Factor Mating Pheromone

Zymo Research, 240 µl/Unit

List Price: $369.11
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Product Overview
  • Aqueous solution of yeast alpha-factor mating pheromone for arresting yeast in G1 of the cell cycle.
Technical Specifications
Amino Acid SequenceTRP-HIS-TRP-LEU-GLN-LEU-LYS-PRO-GLY-GLN-PRO-MET-TYR
Concentration10 mM in 0.1 M sodium acetate pH 5.2, (i.e., 4 mg/240 μl).
Molecular Weight1684.0 Da
Product StorageStore at -20°C. Alpha-factor is stable during multiple freeze-thaw cycles.
Purity> 98% by HPLC
UsageRecommended working concentration: ~5 μM (bar1Δ) to 100 μM (BAR1).
Detailed Description
When yeast 'a' and 'alpha' cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes, affectionately termed 'schmooing'. These alterations prepare the yeast cells for mating and fusion to form stable diploids. The a/alpha diploids are not responsive to mating pheromone of either type, but can be induced to undergo meiosis via nutrient deprivation. The use of yeast mating pheromones has pioneered the study of the cell cycle, cellular morphology, transcriptional induction, as well as signal transduction pathways. Zymo Research provides the alpha-factor peptide mating pheromone as a ready to use liquid that has been optimized for both activity and stability and is guaranteed to retain biological function through multiple freeze-thaw cycles.

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