(11-240) Mix & Go! Competent Cells- Zym Chemically Competent Cells 10 x 100ul/Unit
(11-240) Mix & Go! Competent Cells- Zym Chemically Competent Cells 10 x 100ul/Unit
CAT#: 11-240 Zymo Research

Mix and Go! Competent Cells-Zymo 5a

10^8 - 10^9 transformants/ug, 10 x 100ul/Unit

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Product Overview
  • For general cloning, blue-white screening, plasmid isolation.
  • Mix & Go transformation procedure with transformation efficiencies of 10^8 - 10^9 transformants/µg of plasmid DNA.
  • Simple procedure: add DNA and then spread. DNA transformation in as little as 20 seconds!
Technical Specifications
Additional InfoInsert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.
GenotypeF-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ-
Processing Time20 Seconds
Product Storage-70°C to -80°C
Supplemental Info3 Things to Know for Making Competent Cells
Transformation Efficiency108 - 109 transformants per μg of plasmid DNA
Detailed Description
The Mix & Go E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium - Mix & Go! The premade Mix & Go competent cells are highly efficient (> 108 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Premade Mix & Go competent cells strain Zymo 5a are supplied as a pack of 10 convenient single use aliquots, as a 96-tube rack with removable 8-tube strips, or as a 96-well PCR plate for your high-throughput transformation needs.

Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.

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